TRANSLATIONAL PHYSIOLOGY Differential expression of collagen- and laminin-binding integrins mediates ureteric bud and inner medullary collecting duct cell tubulogenesis
نویسندگان
چکیده
Dong Chen, Richard Roberts, Martin Pohl, Sanjay Nigam, Jordan Kreidberg, Zemin Wang, Jyrki Heino, Johanna Ivaska, Sergio Coffa, Raymond C. Harris, Ambra Pozzi, and Roy Zent Division of Nephrology, Department of Medicine, Department of Pathology, Department of Cancer Biology, Vanderbilt University Medical Center, and Veterans Affairs Hospital, Nashville, Tennessee 37232; Departments of Medicine and Pediatrics, University of California, La Jolla, California 92093; Department of Medicine, Children’s Hospital, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115; and MediCity Research Laboratory and the Department of Medical Biochemistry, University of Turku, Turku, Finland 20520
منابع مشابه
Differential expression of collagen- and laminin-binding integrins mediates ureteric bud and inner medullary collecting duct cell tubulogenesis.
Inner medullary collecting ducts (IMCD) are terminally differentiated structures derived from the ureteric bud (UB). UB development is mediated by changes in the temporal and spatial expression of integrins and their respective ligands. We demonstrate both in vivo and in vitro that the UB expresses predominantly laminin receptors (alpha3beta1-, alpha6beta1-, and alpha6beta(4-integrins), whereas...
متن کاملActivated extracellular signal-regulated kinases are necessary and sufficient to initiate tubulogenesis in renal tubular MDCK strain I cell cysts.
A classic in vitro model of renal cyst and tubule formation utilizes the Madin-Darby canine kidney (MDCK) cell line, of which two strains exist. Most cyst and tubule formation studies that utilized MDCK cells have been performed with MDCK strain II cells. MDCK strain II cells form hollow cysts in a three-dimensional collagen matrix over 10 days and tubulate in response to hepatocyte growth fact...
متن کاملIntegrin-linked kinase regulates p38 MAPK-dependent cell cycle arrest in ureteric bud development.
The integrin-linked kinase (ILK), pinch and parvin ternary complex connects the cytoplasmic tails of beta1 integrins to the actin cytoskeleton. We recently showed that constitutive expression of ILK and alpha parvin in both the ureteric bud and the metanephric mesenchyme of the kidney is required for kidney development. In this study, we define the selective role of ILK in the ureteric bud of t...
متن کاملBeta1-integrin is required for kidney collecting duct morphogenesis and maintenance of renal function.
Deletion of integrin-beta1 (Itgb1) in the kidney collecting system led to progressive renal dysfunction and polyuria. The defect in the concentrating ability of the kidney was concomitant with decreased medullary collecting duct expression of aquaporin-2 and arginine vasopressin receptor 2, while histological examination revealed hypoplastic renal medullary collecting ducts characterized by inc...
متن کاملInduction of collecting duct morphogenesis in vitro by heparin-binding epidermal growth factor-like growth factor.
Heparin-binding epidermal growth factor-like growth factor (HB-EGF), a member of the epidermal growth factor family of growth factors, is synthesized as a membrane-an-chored precursor (proHB-EGF) that is capable of stimulating adjacent cells in a juxtacrine manner. ProHB-EGF is cleaved in a protein kinase C-dependent process, to yield the soluble form. It was observed that HB-EGF acts as a morp...
متن کامل